602panel_test/s1_noUMI_clean_align_20220606.pbs

#!/bin/sh
#PBS -N align_svcalling_noUMI
#PBS -j oe
#PBS -l ncpus=20
#PBS -l nodes=1
#PBS -l mem=20G

#inputpath=$1
#sample=$2
source  /home/liuxiangqiong/miniconda3/bin/activate base
hg19=/cgdata/Database/GATK/b37/human_g1k_v37_decoy.fasta
target=/cgdata/liuxiangqiong/work62pancancer/Client/v0/script/refdata/NanOnco_Plus_Panel_v2.0_Covered_b37_cg.parY2X.sort.bed
#################################################################################1.trim#########################################################
echo "#--------------------------------------STEP1.clean the raw data--------------------------------------#"
fastp -i ${inputpath}/${sample}_R1.fastq.gz -I ${inputpath}/${sample}_R2.fastq.gz  -o ${inputpath}/${sample}_clean_R1.fq.gz -O ${inputpath}/${sample}_clean_R2.fq.gz -c -l 50 -q 20 --umi --umi_loc read1 --umi_len 8 -j ${inputpath}/${sample}.fastp.json -h ${inputpath}/${sample}.fastp.html
echo "#--------------------------------------STEP1.clean the raw data finished!---------------#"
#################################################################################2.mapping#########################################################
#2.1.speedseq align
echo "#--------------------------------------STEP2.align to refdata--------------------------------------#"
speedseq align -t 40  -o ${bam_dir}/${sample}_clean -R "@RG\tID:${sample}\tSM:${sample}\tLB:${sample}" ${hg19} ${inputpath}/${sample}_clean_R1.fq.gz ${inputpath}/${sample}_clean_R2.fq.gz
echo "#--------------------------------------STEP2.align to refdata finished!--------------------------------------#"
rm -rf ${bam_dir}/${sample}_clean.splitters.bam
rm -rf ${bam_dir}/${sample}_clean.splitters.bam.bai
rm -rf ${bam_dir}/${sample}_clean.discordants.bam
rm -rf ${bam_dir}/${sample}_clean.discordants.bam.bai